Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood
نویسندگان
چکیده
منابع مشابه
Detection of Cytomegalovirus (CMV) in Peripheral Blood Specimens in Patients before Keratoplasty
Cytomegalovirus is the most important pathogen affecting transplant recipients and causing significant morbidity and morbidity. The prevalence of CMV infections in transplant recipient varies from country to country. The aim of this study was to determine the prognostic value of PCR assay for CMV detection in patients before Keratoplasty. A total of 23 patient samples en...
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Abstract Background and Objective: Human Cytomegalovirus (CMV) is an important cause of congenital viral infection that can lead to serious diseases and complications in infants. Application of rapid, sensitive, and specific HCMV detection methods is necessary for congenital infection detection. We aimed to optimize the use of PCR and ELISA for detection of HCMV in infants. Material and Methods...
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Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cul...
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Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1995
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.33.5.1411-1413.1995